part conditioned medium (Miltenyi Biotec)
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Miltenyi Biotec
part conditioned medium
Part Conditioned Medium, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+il+2/Human+IL-2+IS%2C+research+grade/pmc13314794-290-18-34
Average 96 stars, based on 133 article reviews
Part Conditioned Medium, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+il+2/Human+IL-2+IS%2C+research+grade/pmc13314794-290-18-34
Average 96 stars, based on 133 article reviews
part conditioned medium - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "In vitro recapitulation of intramuscular mRNA vaccination with naive and recall antigens using a human lymphoid follicle chip platform"
Article Title: In vitro recapitulation of intramuscular mRNA vaccination with naive and recall antigens using a human lymphoid follicle chip platform
Journal: iScience
doi: 10.1016/j.isci.2026.116416
Figure Legend Snippet: Induction of LFs and anti-RABV-G IgG production by SAM-LNP1 in human LF chips (A) Schematic of the LF Chip created with monocytes and conditioned medium from the intramuscular vaccination-mimicking module created with BioRender.com . (B) Representative 3D confocal microscopic stacks showing pseudo-colored follicles (blue) and cell nuclei (green) present within extracellular matrix (ECM) gels cultured for 4 days within a perfused LF Chip when vaccinated with SAM-LNP1 in the absence or presence of IL-2 and IL-4 (SAM-LNP1 + IL-2&IL-4); scale bars, 100 μm. (C) Quantification of the number (left) and size (right) of LFs in LF chips of one donor based on immunostaining followed by confocal imaging. Each data point represents one field of view (left) or an individual follicle (right); 2 independent chips were analyzed per condition. (D) Anti-RABV-G IgG levels in effluents of no treatment LF chips or chips vaccinated with or without IL-2 and IL-4 and cultured for 14 days were detected using a cell-based assay; 3–4 independent chips were analyzed per condition. (E) Quantification of the number (left) and size (right) of LFs in LF chips from four different donors, with each color representing a different donor. Each data point corresponds to one field of view (left) or an individual follicle (right). (F) Anti-RABV-G IgG levels in effluents of LF chips. Each data point represents one chip, with different symbols indicating chips from three independent donors. (G) Anti-RABV-G IgG levels in effluents of LF chips seeded with naive B + bulk T + monocyte mixture. Each data point represents one chip, with different symbols indicating chips from three independent donors. (H) Heatmap showing average log2 fold changes in cytokine levels in the effluents of three LF chips created using bulk lymphocytes from donors whose prior exposure to rabies was unknown, measured using a Luminex Multiplex Assay at various time points (4, 7, 15, 22, and 28 days post-vaccination) compared to the average levels of each cytokine found at day 1. Representative results from one donor are shown in (B)–(D) and (H), with similar outcomes observed in two donors. Data shown are mean ± SD; (C, left; D; and E) one way ANOVA test followed by Tukey’s multiple comparisons test, (C, right; and E) Mann-Whitney U test, (F and G) Welch’s t test.
Techniques Used: Cell Culture, Immunostaining, Imaging, Cell Based Assay, Luminex, Multiplex Assay, MANN-WHITNEY
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Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE/Cy7 anti-CD3 (clone HIT3a) Biolegend Cat# 300316, Lot# B366496; RRID: AB_314052 BV605 anti-CD45RA clone HI100) Biolegend Cat# 304134, Lot# B346157; RRID: AB_2563814 PE anti-CCR7 (clone G043H7) Biolegend Cat# 353204, Lot# B355521; RRID: AB_10913813 PerCP/Cy5.5 anti-LAG-3 (clone 11C3C65) Biolegend Cat# 369312, Lot# B335890; RRID: AB_2629755 AF700 anti-PD-1 (clone EH12.2H7) Biolegend Cat# 329952, Lot# B351613; RRID: AB_2566364 APC/Fire 750 anti-TIM-3 (clone F38-2E2) Biolegend Cat# 345044, Lot# B359214; RRID: AB_2632856 Biological samples Human Peripheral Blood Mononuclear Cells, Frozen STEMCELL Technologies Catalog# 70025 PBMCs from anonymised adult lymphoma patients Discarded leukapheresis tubing sets SingHealth Centralized Institutional Review Board (CIRB; approval #2018/2677) Chemicals, peptides, and recombinant proteins Recombinant Human IL-2 IS, |